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KMID : 0366119740020020111
Korean Journal of Applied Microbiology & Bioengineering
1974 Volume.2 No. 2 p.111 ~ p.117
Studies on Naringinase of Mold. (Part 3)

Abstract
Studies were carried out on the practical use of Naringinase and some chracteristics of Naringin solublizing enzme which might hydrolyze naringin to purunin.
Obtained results were as follows.
1. Selected strain for Naringinase producing was identified to be Aspergillus niger S-1 and its naringinase was applied to chinese citron processing to remove the bitter taste.
2. Of the naringinase, naringin solubilizing enzyme was purified on a DEAF-Sephadex A-50 column and crystalized from acetone and ammonium sulfate,
3. Hydrolized naringin which has higher solubility rather than naringin or naringenin were identified by thin layer chromatography.
4. Hydrolyzed naringin and naringin were separatly determinated by ethylacetate extraction and this result was compared with sensory test.
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